bowtie2-build(1)


NAME

   bowtie2-build - wrapper for bowtie2-build-*

SYNOPSIS

   bowtie2-build [options]* <reference_in> <bt2_index_base>

DESCRIPTION

   Bowtie   2   version   2.2.9   by   Ben  Langmead  (langmea@cs.jhu.edu,
   www.cs.jhu.edu/~langmea)

   reference_in
          comma-separated list of files with ref sequences

   bt2_index_base
          write bt2 data to files with this dir/basename

   *** Bowtie 2 indexes work only with  v2  (not  v1).   Likewise  for  v1
   indexes. ***

OPTIONS

   -f reference files are Fasta (default)

   -c     reference sequences given on cmd line (as <reference_in>)

   --large-index
          force  generated index to be 'large', even if ref has fewer than
          4 billion nucleotides

   -a/--noauto
          disable automatic -p/--bmax/--dcv memory-fitting

   -p/--packed
          use packed strings internally; slower, less memory

   --bmax <int>
          max bucket sz for blockwise suffix-array builder

   --bmaxdivn <int>
          max bucket sz as divisor of ref len (default: 4)

   --dcv <int>
          diff-cover period for blockwise (default: 1024)

   --nodc disable diff-cover (algorithm becomes quadratic)

   -r/--noref
          don't build .3/.4 index files

   -3/--justref
          just build .3/.4 index files

   -o/--offrate <int>
          SA is sampled every 2^<int> BWT chars (default: 5)

   -t/--ftabchars <int>
          # of chars consumed in initial lookup (default: 10)

   --threads <int>
          # of threads

   --seed <int>
          seed for random number generator

   -q/--quiet
          verbose output (for debugging)

   -h/--help
          print detailed description of tool and its options

   --usage
          print this usage message

   --version
          print version information and quit





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